Science, Том 292John Michels (Journalist) American Association for the Advancement of Science, 2001 |
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Стр. 1911
... transfected with plasmid DNA expressing the indicated p53 proteins . Twenty hours after transfection , cells were fused with MDM2 - p53 / - MEF cells while de novo protein synthesis was inhibited . Forty - five minutes after fusion ...
... transfected with plasmid DNA expressing the indicated p53 proteins . Twenty hours after transfection , cells were fused with MDM2 - p53 / - MEF cells while de novo protein synthesis was inhibited . Forty - five minutes after fusion ...
Стр. 2047
... transfected into 3T3 - fibroblast cells ( 3T3 ) and fibroblasts stably transfected with the Ga - coupled serotonin recep- tor 5HT , Normal fibroblast transfectants demonstrate predominant membrane staining , whereas 5HT2 , stable cells ...
... transfected into 3T3 - fibroblast cells ( 3T3 ) and fibroblasts stably transfected with the Ga - coupled serotonin recep- tor 5HT , Normal fibroblast transfectants demonstrate predominant membrane staining , whereas 5HT2 , stable cells ...
Стр. 2049
... transfection reagent ( Qia- gen301425 ) . All other cells were transfected with Effectene . Twenty - four hours after transfection , cells were rinsed once in phosphate - buffered saline ( PBS ) prewarmed to 37 ° C , incubated for 20 ...
... transfection reagent ( Qia- gen301425 ) . All other cells were transfected with Effectene . Twenty - four hours after transfection , cells were rinsed once in phosphate - buffered saline ( PBS ) prewarmed to 37 ° C , incubated for 20 ...
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