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Стр. 1015
The amounts of protein added were in the range in which rate was a linear function of enzyme amount . This range of protein was up to 5 ug / ml for the secreted fraction or 200 mg / ml for the pellet or intracellular fractions .
The amounts of protein added were in the range in which rate was a linear function of enzyme amount . This range of protein was up to 5 ug / ml for the secreted fraction or 200 mg / ml for the pellet or intracellular fractions .
Стр. 1016
The two enzyme forms may be part of a temperature for 4 hours , and amoebas mechanism for control of concentration of cyclic adenosine monophosphate . were removed by centrifugation ( 1000g , 4 ° C , 5 minutes ) .
The two enzyme forms may be part of a temperature for 4 hours , and amoebas mechanism for control of concentration of cyclic adenosine monophosphate . were removed by centrifugation ( 1000g , 4 ° C , 5 minutes ) .
Стр. 1017
Samples of purified enzyme of low molecular weight were stored at 4 ° C in 0.1M tris - HCI , pH 7.4 . The coupled assay monitored by absorbance at 340 nm was used . All activities are set relative to the zero - time value observed with ...
Samples of purified enzyme of low molecular weight were stored at 4 ° C in 0.1M tris - HCI , pH 7.4 . The coupled assay monitored by absorbance at 340 nm was used . All activities are set relative to the zero - time value observed with ...
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