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Стр. 1023
Binding was started by the addition of 100 ul ( 500 ug ) EGTA EGTA of 14C - labeled liposomes , prepared from 50 % phosphatidylserine and 50 % phosphatidylethanolamine , and carried out for 10 min . To some Ca2 + -containing samples ...
Binding was started by the addition of 100 ul ( 500 ug ) EGTA EGTA of 14C - labeled liposomes , prepared from 50 % phosphatidylserine and 50 % phosphatidylethanolamine , and carried out for 10 min . To some Ca2 + -containing samples ...
Стр. 1197
Stimulation was terminated by addition into the medium of 1/5 volume of 5x RIPA buffer containing NaF ( 2.5 mM ) and sodium orthovanadate ( 500 MM ) . ( A ) Tyrosine - phosphorylated proteins in total cell extracts .
Stimulation was terminated by addition into the medium of 1/5 volume of 5x RIPA buffer containing NaF ( 2.5 mM ) and sodium orthovanadate ( 500 MM ) . ( A ) Tyrosine - phosphorylated proteins in total cell extracts .
Стр. 1200
( A ) Release of Amax CaMF ( 20 nM ) after the addition of an excess of unlabeled Cam ( ~ 300 nM ) . Ca2 + ( 50 MM ) , CaMF , and CaM kinase ( 18 NM binding sites ) were preincubated with or without ATP ( + ATP or -ATP ) for 60 s .
( A ) Release of Amax CaMF ( 20 nM ) after the addition of an excess of unlabeled Cam ( ~ 300 nM ) . Ca2 + ( 50 MM ) , CaMF , and CaM kinase ( 18 NM binding sites ) were preincubated with or without ATP ( + ATP or -ATP ) for 60 s .
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